Search results for "Muramic acid"
showing 3 items of 3 documents
Carbohydrate dynamics in particle-size fractions of sandy spodosols following forest conversion to maize cropping
2006
International audience; In southwest France, much of the forested land on sandy spodosols has been converted to continuous maize cropping in the last few decades. To evaluate the impacts of this change on soil organic matter properties, we compared total organic C and neutral and amino sugars content in whole soil and particle size separates of two forested, and five related sites that had been either clear-cut for 12 and 18 mo, or cultivated to maize for 4-22yr. Soil carbohydrates accounted for 4-7% of the total organic C across all sites. Soil organic C contents of clear-cut and cultivated sites were only 57-79% of the average value measured in forested sites. Accordingly, carbohydrate co…
A muramidase from Acremonium alcalophilum hydrolyse peptidoglycan found in the gastrointestinal tract of broiler chickens
2021
Abstract This study evaluates peptidoglycan hydrolysis by a microbial muramidase from the fungus Acremonium alcalophilum in vitro and in the gastrointestinal tract of broiler chickens. Peptidoglycan used for in vitro studies was derived from 5 gram-positive chicken gut isolate type strains. In vitro peptidoglycan hydrolysis was studied by three approaches: (a) helium ion microscopy to identify visual phenotypes of hydrolysis, (b) reducing end assay to quantify solubilization of peptidoglycan fragments, and (c) mass spectroscopy to estimate relative abundances of soluble substrates and reaction products. Visual effects of peptidoglycan hydrolysis could be observed by helium ion microscopy an…
SERS detection of cell surface and intracellular components of microorganisms using nano-aggregated Ag substrate
2016
Abstract The intracellular and cell surface composition and structural features of gram-positive and gram-negative bacteria were identified using near-infrared surface-enhanced Raman scattering (SERS). The structural differences of components that reside in the cell envelope are manifested by their SERS spectra, e.g. gram-negative vs. gram-positive. Silver particles were used as a SERS substrate by exploiting the existence of strong local electromagnetic fields (hot spots) within nanoscale aggregates of the particles. The aggregation of silver nanoparticles was induced by magnesium ions. These hot spots reduce the screening length of the double layer. The obtained SERS spectra showed excell…